Leaf morphology and isozyme profile of Dischidiopsis luzonica Schltr. and Dischidia sp. (Asclepiadaceae)

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

EVANGELISTA, DIANNE ALIBUDBUD. April 2008. Leaf Morphology and Isozyme Profile of Dischidiopsis luzonica SeibItr. and Dischidia sp (Asclepiadaceae). University of the Philippines Los Banos. BS Biology major in Genetics Major Adviser: Prof. Celia B. dela Vifia Co- adviser: Dr. Simeona V. Siar ABSTRACT Dischidiopsis luzonica Schltr, seven Dischidia species (D. acutifolia, D. lancifolia, D. major, D. oiantha, D. platyphylla, D. rosea, and D. vidalli) and four unknown Dischidia species were subjected to leaf morphological characterization and isozyme analysis to assess their genetic diversity. NTSYSpc-version 2.11s by SAHN clustering was used to construct dendrograms based on qualitative, quantitative, and isozyme analysis data. For qualitative characters like mature leaf color, blade, apex, and base shape, margin, upper and lower surface pubescence, midrib and venation appearance and curvature, eleven clusters were formed at 0.72 similarity index the least related species was found to be a specimen of D. vidalli Seven clusters at 1.35 Euclidian distance were formed using quantitative characters like leaf blade length, width, petiole length, leaf area and ratio of length to width, with a specimen of D. platyphylla as the least related species. Isozyme analysis revealed 10 putative loci for the four enzymes used: three each for ALP, ACP, EST, and one for GOT. ALP-1, ACP-1, and EST-1 and GOT- 1 were found to be polymorphic. Three D. platyphylla specimens exhibited distinct handing patterns in comparison to the rest of the species. Five major clusters were formed at 0.63 similarity index coefficient with D. major, and two unknown Dischidia sp. as the least related species. Close affinity was established by D. acutifolia and D. lanciiblia while D. vidalli, D. platyphylla, and D. oiantha were observed to be closely related to D. luzonica.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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