Ronald Allan M Fabella

Date

3-1995

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Desiree M. Hautea

Abstract

ABSTRACT FABELLA, RONALD ALLAN M., University of the Philippines at Los Banos, March 1995. Determination of the Acute Oral Toxicity of the Bruchid Resistance Factor from Vigna radiate var. sublobata Acc. TC1966 on Male Sprague-Dawley Rats. Adviser: Dr. Desiree M. Hautea The crude extracts of VC1973A-R, VC1973A-S and TC 1966 were evaluated for acute toxicity. VC1973A-R and VC1973A-S represent a pair of near isogenic lines (NILs) of V. radiate for bruchid resistance factor while Acc. TC1966 (V. r. var sublobata) is the parent source of this factor. Only VC1973A-S does not harbor the bruchid resistance factor. Powdered seeds of VC1973A-R, VC1973A-S and TC1966 were extracted with phosphate buffered saline (PBS) and the resulting test material was administered to male Sprague-Dawley rats at a constant volume of 10 ml/kg body weight (bw). Single doses of various treatments (45 g/kg bw VC1973A-R, 22.5 g/kg bw VC1973A-R, 20 g/kg bw TC1966, 10 g/kg bw TC1966, 45 g/kg bw VC1973A-S and 0 g/kg bw or pure PBS) were given to the animals using the gavage technique. The crude extracts of VC1973A-R, VC1973A-S and TC 1966 did not exhibit lethality even at high dose levels. However, 10 g/kg bw TC1966 crude extracts slowed down the recovery of growth of treated rats but not throughout the 14-day period. Enlarged spleen was observed in VC1973A-S treated rats. Histological evaluation revealed that 20 g/kg bw TC1966 caused severe disintegration of the mucosa. Based on these results, it was concluded that the presence of bruchid resistance factor in VC1973A-R does not confer any form of acute toxicity towards male Sprague-Dawley rats.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS