Inhibition of activity of cell wall degrading enzymes and growth of bacterial wilt pathogen (Pseudomonas solanacearum E. F. Smith) by flavonoids

Date

3-1995

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

ABSTRACT FLORCRUZ, NILO VINCENT DE GUZMAN. University of the Philippines Los Banos. March 1995. Inhibition of Activity of Cell Wall Degrading Enzymes and Growth of Bacterial Wilt Pathogen (Pseudomonas solanacearum E.F. Smith) by Flavonoids Adviser: Dr. Evelyn Mae T. Mendoza The effects of flavonoids on exocellular cell wall degrading enzymes polygalacturonase and cellulase and the growth of Pseudomonas solanacearum were determined. Pure culture of P. solanacearum was grown on minimal liquid medium for 48 hours at room temperature. The culture was centrifuged; the supernate was removed and dialyzed and used as source of the enzymes. Polygalacturonase enzyme was significantly inhibited by the seven flavonoids and p-coumaric acid, a simple phenolic. The most effective inhibitor was quercitin, a flavonol with ICso = 7 µM while the least effective was apigenin, a flavone with IC,. = 175.92 uM. In the same way, cellulase enzyme was also significantly inhibited by the seven flavonoids and p-coumaric acid. However, the most effective inhibitor was taxifolin with IC, = 25.0 uM and the least effective inhibitor was catechin with only 77.18% inhibition at 100 uM. The potency of the inhibitors was found to be different to both enzymes.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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