Genotypic response of thirteen Philippine seedboard cultivars of rice (Oryza sativa L.) with varying amylose content to callus induction and plant regeneration

Date

4-1995

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Teresa B. Peralta

Abstract

ABSTRACT Flores, Ma. Fatima L. . B.S. Biology, Major in Genetics. University of the Philippines Los Ballos. Genotypic response of thirteen Philippine Seedboard cultivars of rice (Oryza sativa with varying amylose content to callus and plant regeneration. Major Adviser : Dr. Merlyn S. Mendioro Co - Adviser Teresa B. Peralta Genotypic responses of thirteen Philippine Seedboard rice cultivars differing in amylase content on callus formation and plant regeneration were determined. Seed explants from each cultivar were inoculated on basal MS medium (Murashige and Skoog, 1962) supplemented with (a) 2,4-D only and (b) 2,4-D in addition with plant hormones kinetin and NAA. Those from the high amylose group produced calli earlier than those cultivars with low amylase content. Statistical analysis show that differences in response to callus induction are very highly significant with regards to the variety and medium used. Callus induction was observed to be highest in varieties with high amylose content. Callus with various morphological characteristics was likewise observed. Green and compact calli resulted to high rate of plant regeneration.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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