Peroxidase and Phenylalanine Ammonia-Lyase (PAL) in java black rot-infected sweet potato root [Ipomoea batatas (L.) Lam.]

Date

3-1987

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae Tecson-Mendoza

Abstract

ABSTRACT LAWRENCE L. ILAG, University of the Philippines at Los Banos. Bachelor of Science in Biology. March 1987. Peroxidase and Phen lalanine Ammonia-L ase (PAL) in Java Black Rot-Infected Sweet Potato Root /—Ipomoea batatas (L.) Lamj. Adviser: Dr. Evelyn Mae Tecson-Mendoza The study was conducted to determine the effect of slicing and infection by the Java black rot fungus Diplodia tubericola on the activity of peroxidase and phenylalanine ammonia-layse (PAL) in sweet potato root; and from the observed activities, to infer the physiological functions of these two enzymes as related to the resistance mechanism of sweet potato. Peroxidase and PAL activity of sliced and D. tubericola-infected roots of resistant (R) G81r-8a and the susceptible (S) G52-5 sweet potato varieties were thus spectrophotometrically determined after 0, 1, 3, and 5 days of incubation. Generally, peroxidase and PAL activity in all root tissues (i.e., G81r-8a sliced and infected, G52-5 sliced and infected) increased. No significant difference in peroxidase activity was observed between infected G81r-8a (R) (15.57 units/mg after 3 days of incubation) and infected G52-5 (S) (14.39 units/mg), and sliced G81r-8a (9.32 units/mg) and sliced G52-5 (9.05 units/mg). Meanwhile,

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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