Immunochemical characterization of seed proteins of five Vigna species

Date

3-1985

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dolores A. Ramirez

Abstract

ABSTRACT ILAG, LEODEVICO L. University of the Philippines at Los Banos, March, 1983. Immunochemical Characterization of Seed Proteins of Five Vigna Species. Adviser: Dr. Dolores A. Ramirez

Antisera against Vigna radiata and Vigna umbellata crude protein (mostly globulins) were raised from three young albino rabbits in a period of 3 months. Crude protein (mostly globulins) extracts from V. radiata, V. angularis, V. umbellata, V. ungui-culata and V. mongo were cross-reacted on agar plates with anti-radiata and anti-umbellata using modified Ouchterlony double diffusion to test for antigenic similarities among the different species. Double diffusion test revealed different types of cross-reactions among V. radiata, V. angularis, V. umbellata, V. ungui-culata, and V. mongo against anti-radiata and anti-umbella. Results show that V. angularis, V. unguiculate, and V. umbellata are antigen-rich, i.e. they consistently form many precipitin lines of different types against the antisera. A hypothesis was proposed that V. angularis diverged earlier from the evolutionary lines of V. unguiculata and V. umbellata as observed from immunochemical data. Consistent relationships were seen among V. unuiculata, V. mongo, V. radiata, and V. umbellata relative to anti-umbellata and anti radiata. Similar strong homologies were observed between V. unguiculata and V. mongo and between V. mongo and V. radiata

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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