Isolation and characterization of water-soluble colorants from Bixa orellana Linn. seeds

Date

10-1985

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

Colorants in carbon tetrachloride, ethyl ether, and chloroform were obtained from the crude water extract of Bixaorellana Linn. seeds by rapid column chromatography. The ether fraction components were further isolated by thin layer chromatography. Spectral data gave valuable information about functionalities present in all the isolates but did not allow full elucidation of structures of the colorants. The ultraviolet-visible spectra suggested extensive conjugated polyene chains as the main chromophores of the isolates with ketones which were not conjugated to these polyene chains. The infrared spectra of all the isolates had indications of the presence of methyl and methylene groups, olefins, and confirmed the presence of ketones. The signals in the nuclear magnetic resonance spectra using deuterated chloroform as solvent were quite weak and did not give additional information on the nature of the isolates.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1985 C4 I36

Document Type

Thesis

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