The inheritance of seed coat color in mungbean (Vigna radiata (L.) Wilczek)

Date

3-1991

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dolores A. Ramirez

Abstract

ABSTRACT ILAG, LEOPOLD LUNA. UNIVERSITY OF THE PHILIPPINES AT LOS BANOS. MARCH 1991. PATTERN OF SEED COAT COLOR INHERITANCE IN MUNGBEAN Vigna radiata (L.) Wilczek

Professor: DR. DOLORES A. RAMIREZ

-The inheritance of seed coat color in mungbean, Uj„nna radiata (L.) Wilczek, was determined using both visual and biochemical tests. Seed coats of three generations namely the green parentals (Acc 163), yellow parentals (Acc 1), F1 (green) and F2's (yellow, dull green and shiny green) were analyzed to identify the pigments present. Upon extraction of the pigments, the resulting aqueous and organic layers were subjected to thin layer chromatography, UV fluorescence test and spectrophotometric analysis. The green parents contain chlorophyll a and chlorophyll h, and beta or alpha carotene in addition to luteolin and apigenin. The yellow parent had alpha or beta carotene. Furthermore it contains quercetin glycoside-3-arabinoside,quercetin-3- glucoronide and luteolin in the aqueous layer. Analysis of the water soluble pigments in Fl progenies showed a pigment which was not present in either parent suggesting gene interaction. Both the shiny and dull green progenies contained chlorophyll a and b, xanthophyll and alpha carotene as well as the three pigments present in the aqueous layer of the yellow parent showing that luster is independent of the pigments present. Analysis of the aqueous layer revealed the inheritance of quercetin-3-glucoronide by the dull and shiny green progenies from the yellow parent The F2 segregation ratio of 3:1 indicate a monogenic difference between the green and yellow seeded parents and the complete dominance of green over yellow seed coat color in mungbean.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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