Morpho-cytological response of the unicellular green alga, Chlorella SP. to the different concentrations of the herbicide, Butachlor

Date

10-2003

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Milagrosa, R. Martinez-Goss

Abstract

KAMPITAN. VINCI ANN B. 2003. Morpho-cytological Responses of the Unicellular Green Alga, Chlorella sp. to the Different Concentrations of the Herbicide, Butachlor. University of the Philippines Los Banos. B.S. Biology Major in Cell Biology. Adviser: Dr. Milagrosa R. Martinez-Goss ABSTRACT Morpho-cytological responses of the unicellular green alga, C'hlorella sp., to the different concentrations of the herbicide, butachlor, were determined under laboratory conditions. Unialgal culture of Chlorella sp. was subjected to eight treatments during its logarithmic phase of growth and was exposed to increasing concentration of the herbicide, butachlor, such as: TI (10ppm), T2 (0.001ppm), T3 (0.01ppm), T4 (0.1ppm), T5 (1ppm), T6 (5ppm), T7 (10ppm), and T8 (20ppm). The range of the cell diameter varied at different butachlor concentrations. T1 (control) showed the highest average cell diameter (4.325m). The smallest diameter (3.088m) was noted in T5 (1ppm). Chlorella cells exhibited spherical to ellipsoidal shapes at lower concentrations (Tl , 0ppm up to T5, 0ppm of butachlor). While at higher concentrations (T6, 5ppm up to T8, 20ppm) the cells were mostly spherical. Only the cells under the control condition maintained a grass-green color while the rest of the cells at different treatments showed yellow green color. There was an effect on the chloroplast of Chlorella cells exposed to increasing concentrations of the herbicide, butachlor. At lower concentrations, T1, T2, and T3, a normal cup-shaped chloroplast was noted and a dividing cell was noticed in T2. A slight decrease in the size of the chloroplast was observed in T4 and T5. Higher concentrations of butachlor exemplified by T6, T7, and T8 showed fragmentation of the chloroplast, reduced chloroplast size, which was found almost on the periphery of the cell, and disruption of some cells. Different concentrations of butachlor affected the morpho-cytology of Chlorella sp. and the degree of changes observed on the chloroplast could be the best indicator to detect the presence or absence of butachlor in aquatic ecosystems.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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