"Genetic diversity of seven Solanum species in the Asian Vegetable Rese" by Julius Cesar G. Laban

Genetic diversity of seven Solanum species in the Asian Vegetable Research and Development Center (AVRDC) gene bank based on Random Amplified Polymorphic DNA (RAPD) markers

Date

3-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

LABAN, JULIUS CESAR G. 2003. Genetic Diversity of Seven Solanum Species in the Asian Vegetable Research and Development Center (AVRDC) Gene Bank Based on Random Amplified Polymorphic DNA (RAPD) Markers. University of the Philippines Los Banos. B. S. Biology major in Genetics.

Adviser: Prof. Ivan Marcelo A. Duka

ABSTRACT Random amplified polymorphic DNA (RAPD) markers were used to determine the degree of variation among seven Solanum species maintained at the Asian Vegetable Research and Development Center (AVRDC) gene bank, namely: S. americanum Mill. (American black nightshade), S. aethiopicum L. (African scarlet eggplant), S. incanum L. (Palestine nightshade), S. macrocarpon L. (Gboma eggplant), S. sisymbriifolium Lam. (Sticky nightshade), S. torvum Sw. (Turkey berry), and S. villosum Mill. (African nightshade). A total of 32 accessions from seven Solanum species were used in the study. From the screening of 869 primers, eight and 45 primers were selected for the intra-accession and inter-accession studies, respectively. The intra-accession study showed the homogeneity of individuals per accession. For the inter-accession study, 252 RAPDs were scored from the amplification products of 15 randomly selected oligomer primers. An average of 17 amplification products were scored for each primer and possible markers were noted for each species. A S. villosum accession from Bangladesh (S00810) was observed to be a S. americanum accession based on RAPD markers. In spite of this, further tests should be conducted to confirm this observation. Jaccard's similarity coefficients ranged from 0.02 to 1.00 indicating a high level of genetic diversity. The cluster analysis grouped 24 out of 32 accessions in six close-knit clusters (similarity? 0.60). The b ootstrap estimates of confidence limits were significant for six out of 30 nodes in the majority-rule consensus tree (>95% occurrence). The results of both analyses showed the same composition for each cluster. The high degree of variation among the seven .Solanum species can be attributed to their inherent variation and geographical distribution. Therefore, RAPD assay is useful in germplasm characterization of Solanum species. However, more primers can be used to further elucidate the identity and genetic relationships of the seven Solanum species used in the study.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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