"Comparative changes in activity of bacterial pectate lyase and protease in resistant and susceptible tomato (Lycopersicon esculentum Mill.) in response to the bacterial wilt pathogen (Pseudomonas solanacearum E. F. S.)"

Date

4-1993

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae Tecson-Mendoza

Abstract

LAPIS, CHARISSE TUCAY. University of the Philippines Los Banos. March 1993. Comparative Changes in Activity of Bacterial Pectate Lyase and Protease in Resistant and Susceptible Tomato (Lycopersicon esculentum Mill.) In Response to the Bacterial Wilt Pathogen (Pseudomonas solanacearum E.F.S.) Adviser: Dr. Evelyn Mae T. Mendoza

Biochemical changes in both resistant and susceptible tomato plants were analyzed in terms of changes in activity of pectate lyase and protease. For pectate lyase, enzyme activity in the roots of resistant plants increased by 61.3% and decreased by 40% in the late stages of infection. The same trend was seen in the susceptible plants but activity was higher by 23-56% than the resistant. For the resistant stems, there was an 85% increase from day 1 to day 5 and a decrease of 33% in day 7. The susceptible line also followed this trend but enzyme activity was higher by 14-83%. The leaves also followed the same pattern but activity was again higher in the susceptible by 22-60%. Enzyme activities in the different components showed the highest level in the leaves followed by the stems and then the roots. In terms of protease activity, an increase of 73% and 64% was seen from the first day of infection until the last day of the roots of both resistant and susceptible varieties, respectively, but activity was higher in the susceptible by 9-36%. An increasing trend was also seen in the stems both for resistant (74%) and susceptible 76% but activity in the susceptible is higher by 5-28%. Likewise in the leaves, protease increased until the late stages of wilting but activity was higher in the susceptible by 4-41%. Comparison of activity in the different plant components showed highest activity in the leaves.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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