Determination of the maximum concentration of sambong (Blumea balsamifera L.) DC. tablets tolerated by adult human leukocytes cultured in vitro based on cytogenetic effects

Date

3-1999

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

LAPITAN, DELINIA G University of the Philippines at Los Banos March 1999 Determination of the Maximum Concentration of Sambong Blumea balsarnifera (L.) DC. Tablets Tolerated by Adult Human Leukocytes Cultured in vitro based on Cytogenetic Effects. Undergraduate Thesis B S Biology (Genetics ,

Adviser' Dr. Merlyn S Mendioro

Leukocytes from seven randomly-selected adult males (aged 30 to 60 years) were cultured and treated with three concentrations of sambong (Blumea balsamifera) tablet - 2%, 3% and 5%. while one culture served as control receiving only sterile saline solution Analysis of variance showed that there was a significant decrease in the mitotic indices control = 0.1226. 2% = 0,0799, 3% = 0 0721 and 5% = 0 0417 with the 2% and 3% treatments having no significant difference between them according to Duncan's Multiple Range Test (DMRT). No significant differences on the mean frequencies of chromosomal aberrations namely gaps (control = 0.0149, 2% = 0.0223. 3% = 0 0171 5% = 0 0219), breaks (control = 0 0143, 2% = 0 0054. 3% = 0 0143 5% = 0 0190) loose sister chromatids (control = 0.0435, 2% = 0 0573. 3% = 0 0442, 5% = 0 0686) and condensed chromosome sets (control = 0 0892, 2% = 0 0955, 3% = 0 0927, 5% = 0 1162) were observed Based on these results it is recommended that sambong tablets should only be taken in dosages below 2% to avoid any harmful effects.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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