Validation of salinity tolerance quantitative trait loci-linked simple sequence repeat markers in chromosome 1 in selected salt tolerant genotype of rice (Oryza sativa L.).
Date
4-2011
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Neilyn O. Villa
Co-adviser
Glenn B. Gregorio
Abstract
LAPITAN, JONA YOANA CHAVEZ. 2011. VALIDATION OF SALINITY 'TOLERANCE QUANTITATIVE TRAIT LOCI-LINKED SIMPLE SEQUENCE REPEAT MARKERS IN CHROMOSOME I IN SELECTED SALT TOLERANT GENOTYPES OF RICE (Oryza saliva L.). University of the Philippines Los Banos. BS Biology Major in Genetics.
Adviser: Neilyn 0. Villa Co-adviser: Glenn B. Gregorio
ABSTRACT
Three hundred and nine (309) randomly selected lines of rice (Oryza saliva L.) were evaluated for their salinity tolerance levels at seedling stage using hydroponic solution. Of the selected lines, 57.28% were scored as susceptible to salinity stress. SSR markers were used to evaluate the genetic diversity of 37 selected genotypes from the original 309 lines. A total number of 20 alleles were detected. RM493 exhibited the highest PIC value of 0.6612 while RM315 attained the least (0.3267). The mean PIC value of 0.4480 indicated the markers' limited ability to detect genetic variability in the genotypes. A dendrogram was constructed to visualize the relatedness of one genotype from another. Due to the limited number of SSR markers, no consistent pattern was observed in the dendrogram. Haplotype analysis was done to validate the presence of QTL-linked SSR markers for salt tolerance in chromosome 1 among the selected genotypes. Three markers, namely RM3412, RM493 and RM140, were found to produce bands that were also present in the tolerant reference line (Pokkali). Utilization of more markers is recommended to establish better relationships between genotypes and to efficiently study the genetic variation among the samples.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Lapitan, Jona Yoana C., "Validation of salinity tolerance quantitative trait loci-linked simple sequence repeat markers in chromosome 1 in selected salt tolerant genotype of rice (Oryza sativa L.)." (2011). Undergraduate Theses. 11194.
https://www.ukdr.uplb.edu.ph/etd-undergrad/11194
Document Type
Thesis
