Cytogenetic effect of heroin on chromosomes of human leukocytes in vitro

Date

3-1979

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dolores A. Ramirez

Abstract

Susan M. Madrid. University of the Philippines at Los Banos. The Cytogenetic Effect of Heroin on Chromosomes of Human Leukocytes In Vitro.

Adviser: Dr. Dolores A. Ramirez

The effects of heroin concentrations (0.025%, 0.050% and 0.075%) were studied using leukocyte cultures obtained from five males and five females whose ages range from 19 to 20 years old. The number of aberrant cells in groups treated with heroin showed statistically significant deviation from the control groups. Increased frequencies of aberrant chromosomes were observed with increased concentration of the drug, showing dose dependency of the action of the drug. Analysis of the data showed that there was no significant differences bet-ween the two age groups and also between male and female leukocytes in terms of chromosomal aberrations. The groups of larger chrohnosomes were more affected at every concentration. The aberrations observed are chromatid and isochromatid gaps and breaks and loosely paired sister chromatids. Terminal deletions, minutes or intercalary deletions and acentric rings were grouped as acentric fragments. The probable factors and mechanisms for the observed abnormalities were discussed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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