Morphological characterization and isozyme analysis of eight Syzygium species

Date

4-2007

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Co-adviser

Felipe S. Dela Cruz, Jr.

Abstract

MAGUAD, APRIL ANNE T. 2007. Morphological Characterization and Isozyme Analysis of Eight Syzygium Species. University of the Philippines Los Banos. BS Biology major in Genetics.

Adviser: Celia B. Dela Vifia Co-adviser: Felipe S. Dela Cruz, Jr.

ABSTRACT

Morphological characterization and isozyme analysis were done on eight species of Syzygium, namely: S. aqueum (pink and white water apple), S cumini (giant, Sta. Fe, common, and seedless duhat), S. curranii (lipote), S tripinnatum (hagis), S jambos (tampoi), S. malaccense (yambu), S. polycephaloides (baligang) and S. samarangense (makopa maroon, green, pink, and white and tambis). Leaf, fruit, and seed qualitative and quantitative morphological characteristics such as leaf shape and length, fruit color and weight, and seed shape and number, revealed consistent pattern of relationships and clustering of the eight species of Syzygium from the constructed dendrograms using NTSYSpc by SAHN clustering. These confirm close affinity between accessions of S. aqueum, among accessions of S. cumini, among accessions of S. samarangense and between S. curranii and S. polycephaloides. Shannon-Weaver diversity index was used to determine the extent of diversity of the Syzygium species being conserved at the field genebank of 1PB. From the 19 qualitative traits analyzed, 12 were found to have high diversity index with fruit shape having highest value (std. H'=0.91). Mature leaf color (std. H'.37), fruit skin texture (std. H'=0.37), and shape of leaf margin (std. H'=0.35) had low diversity index. High diversity indexes were obtained in all the quantitative characters analyzed. Isozyme analysis yielded 15 putative loci encoding for six enzymes: two loci each for acid phosphatase (ACPH), malate dehydrogenase (MDH), phosphoglucomutase (PGM), and shikimate dehydrogenase (SDH), three for alkaline phosphatase (ALPH), and four for esterase (EST). ACPH-I, ACPH-2, ALPH-2, MDH-2, and SDH-2 loci were found to be polymorphic. Five possible enzyme systems were able to distinguish a certain Syzygium species from the rest of the eight species based on unique banding pattern exhibited—ACPH (S. samarangense (makopa white) and S. malaccense), ALPH (S. samarangense (makopa white) and S. tripinnatum), EST (S. aqueum (pink water apple) and S. tripinnatum), MDH (S. samarangense (makopa green) and S. curranii), and SDH (S. cumini (common duhat)). Each of the four accessions of S. cumini (duhat) also yielded unique banding patterns for EST and SDH. Dendrogram confirmed the similarity of all the Syzygium species at 0.54 and four major clusters were observed at approximately 0.75 similarity index. S. tripinnatum was found to be the least related.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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