C- and pancreatin G- banding of patterns of human mitotic chromosomes

Date

6-1998

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

MALAPASCUA, JOSE ROMEL F. University of the Philippines at Los Banos, Laguna; B.S. Biology, June 1998. C- and Pancreatin G- Banding Patterns of Human Mitotic Chromosomes. Adviser : Dr. Adelina A. Barrion Characteristic banding patterns of darkly stained regions separated by pale regions along the longitudinal lengths of the human mitotic chromosomes were observed after subjecting the chromosome preparations to modified C- and G- banding techniques. Karyotypes of these banding patterns were constructed according to Bregman (1990) utilizing photomicrographs of C-metaphase spread of C- banded (Category 1 chromosomes) and Pancreatin C-banded (Category 3 chromosomes) human mitotic chromosomes focused under phase-contrast microscope, interpretative drawings, chromosome lengths and band homology. Results showed that both banding techniques rely heavily on Giemsa to reveal AT-rich regions which are highly condensed during interphase. Specifically, C-bands are repetitive DNA sequences located near or on flanking centromeric regions of the chromosomes. G-bands, on the other hand, are moderately repetitive DNA sequences located in intercalary heterochromatic region of the chromosomes. Also, locally established C- and Pancreatin G- banding patterns of human mitotic chromosomes when compared to previously established C- and G-banding patterns revealed that constitutive heterochromatin located at the centromere were preferentially stained in C-banding while intercalary heterochromatin dispersed along the chromosome arms were stained in 0-banding. Discrepancies observed were due to different conditions of the chromosomes used and that overlapping and overcrowding of chromosomes affect banding patterns.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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