Isozyme polymorphism in the native orchids Pomatocalpa sp., Renanthera sp., and Trichoglottis sp.

Date

4-2000

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

MALLARI, NAPOLEXANDER Q. University of the Philippines Los Banos, March. 2000. Isozyme Polymorphism in the Native Orchids Pomatocalpa sp., Renanthera sp., and Trichoglottis sp..

ABSTRACT

Enzyme polymorphism in the native orchids Ponaloca1pa sp., Renanthera sp., ;and Trichoglottis sp. was analyzed by comparing the banding patterns of the enzymes acid phosphatase (Acph), alkaline phosphatase (Alph), esterase (Est), and malate dehydrogenase (Mdh) using starch gel electrophoresis. A total of seven presumptive loci were observed in the three species, three loci for alkaline phosphatase, two for acid phosphatase, one for Esterase, and one for Malate dehydrogenase. Polymorphism was observed in the two enzymes alkaline phosphatase and acid phosphatase. Malate dehydrogenase and Esterase were both monomorphic for the three species. In terms of presumptive allelozymes in each locus, S for slow migrating bands and F for fast migrating bands were observed in Alph-1, Alph-2, Alph-3, Acph-1, and Acph-2. The three species were also monomorphic for S in the Est and Mdh loci. The degree of genetic identities, genetic distance, and genotypic similarity were computed for the three native orchid species. It was inferred that they still share a high percentage of common alleles based on the enzyme systems studied. The three species may have different genotypic frequencies but they have similar allelic frequencies.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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