Screening, isolation and partial characterization of galactomannans from seeds of selected legumes

Date

7-2000

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

Seeds of five selected legumes were screened for galactomannan. These include fire tree (Delonix regia), golden shower (Cassia fistula), batao (Dolichos lahlab), sabawel (Mucuna pruriens) and samsamping (Clitoria ternatea). Of the five samples, fire tree and golden shower exhibited precipitation with Fehling's solution, indicating the presence of galactomannan. Isolation of the galactomannan from these two types of seeds yield an average of 41 % and 4 % for fire tree and golden shower. respectively, based on the air dry weight of starting material. Qualitative analysis of the isolated polysaccharide using solutions of ninhydrin and starch confirmed that the isolated polysaccharide was free from protein and starch. Viscosity analysis using the Brookfield viscometer at 0.10 % solution showed that polysaccharide from fire tree has a kinematic viscosity of 20.025±0.002 cent:poise and for golden shower polysaccharide, 23.50±0.020 centipoise. All of the values were compared to the kinematic viscosity of guar, a standard source of galactomannan, which is 20.05±0.002 cent:Poise. Average intrinsic viscosity of the isolated polysaccharide from fire tree was 1.0370.12 dUg. Hydrolysis of the isolated polysaccharide from fire tree using 50% H,SO4 for 15 hours and dilution of hydrolyzate to water (1:10, v/v/v) gave at least mama= and galactose . Thin layer chromatography using 1-butanol-ethanol-water (4..1:5,v/v/v) yielded two spots with Rf values of 0.27 and 0. 35, corresponding to the Rf values of D-p lactose and D-mannose standards, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2000 A13 M35

Document Type

Thesis

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