Sequence analysis of two forms of a-D-galactosidase in rice (Oryza sativa L.) and developing endosperm of coconut (Cocos nucifera L.)
Date
4-2009
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Maria Genalee Q. Diaz
Abstract
Two forms of a-D-galactosidase were amplified, cloned, and sequenced using rice and coconut DNA as templates. PrimerBlast was used to design primer pairs based on the genome sequence of Oryza swim subspecies japonica since rice genome is considered as the model sequence for monocots. No PCR product was observed in coconut and expectedly, —950bp and —900bp products were amplified for the rice a-galactosidase and alkaline a-galactosidase, respectively. The BLASTN search revealed 100% query coverage and 0.0 E value with rice's (0s01 g0518500) and (0s06g0172800) for a-galactosidase and alkaline a-galactosidase, respectively. BLASTP of the deduced amino acid sequences of both a-galactosidase and alkaline a-galactosidase showed high degree of similarities with other a-galactosidase and alkaline a-galactosidase from other plant species, mostly monocots. Comparison between the a-galactosidase and alkaline a-galactosidase revealed that their nucleotide and amino acid sequences are not highly similar.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Manzano, Mark R., "Sequence analysis of two forms of a-D-galactosidase in rice (Oryza sativa L.) and developing endosperm of coconut (Cocos nucifera L.)" (2009). Undergraduate Theses. 11282.
https://www.ukdr.uplb.edu.ph/etd-undergrad/11282
Document Type
Thesis