Response of dendrobium "Jacquelyn Thomas Pink" to gamma rays and colchicine.

Date

3-1994

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita L. Rosario

Abstract

The response of the protocorms from the embryo culture of Dendrobium 'Jacquelyn Thomas Pink' to gamma rays was studied. Protocorms from embryo culture were reflasked and irradiated with 0.5 kr, 1.0 kr, 2.0 kr and 3.0 kr with eight replicates including the control. Analysis of variance showed non-significant differences between the control and the treated protocorms. However, Duncans' Multiple Range Test obtained significant differences in plant height, root length and root number within treatments while non-significant difference was shown for leaf number.

When the irradiated plants were reflasked to fresh media containing 0.05% colchicine, appearance of protocorms like bodies and thickening of leaf bases were observed. Colchicine and gamma ray treatment delayed the differentiation of the tissues and exerted certain phenotipic characteristics. The cells obtained from the protocorm-like bodies showed lesser organization and they are larger than normal

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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