Isozyme analysis of cultivated artocarpus (Moraceae)

Date

4-2007

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Isozymes in seven Artocarpus species obtained from the National Plant Genetics Resources Laboratory, Institute of Plant Breeding, UPLB and at the vicinity of Mt. Makiling were analyzed via starch gel electrophoresis. Species include jackfruit (A. heterophyllus Lamarck), monkey jackfruit (A. rigidus Blanco), breadfruit (A. atilis (Parkinson) Forsberg), breadnut (A. camansi Blanco), marang (A. odoratissimus Blanco), gumihan (A. sericicarpus Jarrett) and Artocarpus sp. Five presumptive loci were detected for the enzyme systems acid phosphatase, alkaline phosphatase, and esterase. Two loci were observed for acid phosphatase: monomorphic ACPH-1 (0.08< Rf < 0.13) and polymorphic ACPH-2. Bands observed for the second loci were slow ACPH-25 (0.23 < Rf < 0.30) and fast ACPH-2F (0.35 < Rf < 0.42). Alkaline phosphatase had only one polymorphic locus: ALP-1s (0.17 < Rf < 0.22) and ALP-1F (0.27 < Rf < 0.32). Two loci were detected in esterase: EST-1 (0.10 < Rf < 0.15) and EST-2 (0.26 < Rf < 0.31). From the isozyme data, a dendrogram showing relatedness of the species was constructed. At 0.7 index of similarity, A. odoratissimus and A. heterophyllus were found in one cluster. A. altilis and A. camansi were identical; belonging to another cluster along with Artocarpus sp. and A. sericicarpus. The most distant of the species was A. rigidus. Clustering and relatedness of the species could possibly be attributed their endemicity in the Philippines and their subsequent introduction.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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