Induction of 'Mayana', Coleus blumei Benth. (Family Lamiaceae) tissue culture using three varieties for the elaboration of secondary products

Date

4-2007

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Lourdes B. Cardenas

Abstract

Three Coleus blumei varieties: `Defiance,' Shocking Pink,' and 'Small Lear were studied to determine which is the best explant source for callus induction. Each variety was characterized morpho-anatomically and histochemically. 'Defiance' was found to have the longest trichomes (0.254 mm) while 'Small Leaf' has the highest density of trichomes (200 trichomes per mrn21. All contained polyphenols being most pronounced in 'Defiance'. Based on thin layer chromatography, 'Shocking Pink' has the highest level of photosynthetic pigments, 'Defiance' has the highest level of anthocyanins and 'Small Leaf' has the highest amount of rosmarinic acid. When leaf explants from these three varieties were inoculated in culture media for callus induction, 'Small Leaf' had the highest percent of contamination at 81.4%, 'Defiance' at 75.0%, and `Shocking Pink' at 20.0%. In terms of culture responsiveness, 'Small Leaf' grown on MS medium with 1 ppm BAP and 6ppm 2,4-D, was the most responsive, producing white friable callus on green explant tissue at three weeks after inoculation. 'Shocking Pink' produced brown friable calli with extensive tissue browning due to phenolic oxidation one month after inoculation. 'Defiance' exhibited no significant change in culture even at two months after inoculation. Among the three, 'Small Leaf' proved to be the most promising explant source in inducing C. blumei tissue culture for rosmaric acid production.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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