Isozyme variation in natural populations of Apis cerana F. (Hymenoptera: Apidae from Batangas and Palawan

Date

4-1994

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

MURILLO, MA. THERESA A. Isozyme Variation In Natural Populations of Apis cerana F. (Hymenoptera: Apidae) From Batangas and Palawan.

Major Adviser: Dr. Rita P. Laude

Twenty colonies of A. cerana F. from Batangas and eight colonies from Palawan were analyzed by starch-gel electrophoresis to determine the extent of genetic variability between the two geographically isolated areas. Esterase (Est), malate dehydrogenase (Mdh) and alkaline phosphatase (Alph) were found to be polymorphic while isocitrate dehydrogenase (Idh) was monomorphic. EstS, EstM and EstF genes were observed at a frequency of 0.6218, 0.3122, and 0.0660, respectively for Batangas and 0.5217, 0.4022, and 0.0761, respectively fo Palawan. EstSS, EstSM, EstMM and EstSF phenotypes were observed in the two populations, EstSM being the most predominant form with a frequency of 0.6041 for Batangas and 0.6081 for Palawan. On the otherhand, MdhS, MdhM and MdhF were observed in Batangas at a frequency of 0.2194, 0.7667 and 0.0139, respectively. Same phenotypes were observed in Palawan with a frequency of 0.2778, 0.1667 and 0.5556, respectively. The phenotypes observed in Batangas were MdhSS, MdhSM, MdhMM and MdhMF, MdhMM being the most predominant form with a frequency of 0.6500. The phenotypes in Palawan had an additional MdhSF and MdhFF, MdhFF as the most predominant form with a frequency of 0.4444. However, only the genes AlphlS and AlphlF were observed in the two populations. AlphlS and AlphlF frequencies for Batangas were 0.4543 and 0.5754, respectively while the gene frequencies in Palawan were 0.9571 and 0.0428, respectively. The phenotypes observed in Batangas were Alph1SS, Alph1SF and Alph1FF and only Alph1SF and A1ph1FF were found in Palawan. The most predominant form in Batangas was Alph1SS with a frequency of 0.4628 while in Palawan, the most predominant form was Alph1FF with a frequency of 0.8871. The phenotype frequencies in both populations did not show a goodness of fit to the expected under the Hardy-Weinberg equilibrium except for the Alph found in Palawan. There was a low genetic variability between the natural population of Batangas and Palawan. The genetic identity (I) was 0.8841 and the genetic distance (0) value was 0.0535.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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