Biochemical basis of resistance to Cercospora leaf spot in Arachis hypogaea Linnaeus

Date

3-1987

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dolores A. Ramirez

Abstract

This study was conducted to determine the components of sixteen accessions of peanuts, Arachis hypogaea Linnaeus before and after inoculation with Cercospora personata Berkeley and Curtis. Also the difference in the compounds produced by the susceptible, moderately resistant, and resistant accessions wee deter-mined. Using two dimensional paper chromatography with with 5% acetic acid (vol/vol) as the first solvent and butanol; acetic acid; water (6:1:1), the inoculated plants produced more phenolic compounds than the uninocul7.ted samples, regardless of the disease reaction to cercospora leaf spot. The susceptible accessions, before inoculation, produced six compounds, and after inoculation, nine compounds. The moderately resistant accessions uninoculated samples produced nine compounds while the inoculated samples had eleven. The resistant accession had three compounds before inoculation, Comparison with standards ferrulic acid, p-coumaric acid trans-cinnamic acid, and chlorogenic acid revealed that the susceptible accessions produced chlorogenic acid and p-coumaric acid. The moderately resistant accessions produced chlorogenic acid, ferrulic acid, and trans-cinnamic acid while the resistant accession produced chlorogenic acid. Statistical analysis further revealed that the number of compounds produced by the uninoculated and the inoculated samples differ significantly, at the 1% ; level of significance. The number of compounds produced by the accessions with varying disease reactions, before and after inoculation, also differed significantly. The probable roles of the compounds in resistance of peanuts to Cercospora leaf spot, and the differences in the number of compounds produced by the plants with varying disease reactions to Cercospora leaf spot are discussed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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