"Genetic diversity based on sodium dodecyl sulfate- polyacrylamide gel " by Mae Kirstin C. Oporto

Genetic diversity based on sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-page) of leaf proteins in mangoes, Mangifera sp., from the National Plant Genetic Resources Genebank

Date

4-2002

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

OPORTO, MAE KRISTIN. College of Arts and Sciences, University of the Philippines, Los Banos. April 2002. Genetic Diversity Based on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of Leaf Proteins in Mangoes, Mangifera sp., from the National Plant Genetic Resources Genebank.

Adviser- Ma. Genaleen Q. Diaz

This study was conducted to determine the genetic diversity among 39 National Plant Genetic Resources (NPGR) genebank trees of three different species of Mangifera sp. namely: Mangifera indica L., Mangifera laurina BI., and Mangifera alussima BI using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) A total of 29 bands were resolved. No common band pattern was observed among the different accessions. Only band number 1 appeared in majority of the accessions (77%). In Mangifera indica L., the most common bands were. for carabao, band numbers I, 7 and 21; for pico-like, band numbers 1 and 12; for Hawaiian band numbers 1, 7, 20 and 28; for Thong Dam band numbers 5, 10, 15, 17, 19 and 29, and for other varieties with single accessions, band numbers 1, 12 and 19. Unique bands were observed in some accessions. Similarity indices were generally low for all accessions. Similarity indices within species were higher than between species. All the observations reflect the presence of a wide genetic diversity in the NPGR genebank collection of Mangifera indica L

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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