Galactomannan from seeds of selected Philippines legumes : screening, isolation and partial characterization

Date

1-2002

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Abstract

ABSTRACT

ORCINO, JOHANN S. University of the Philippines Los Banos, January 2002. Galactomannan from Seeds of Selected Philippine Legumes: Screening, Isolation and Partial Characterization.

Adviser: Dr. Evelyn Mae T. Mendoza

Seeds of patani (Phaseolus lunatus) kakawate (Gliricidia sepium), katuray (Sesbania grandiflora), kadios (Cajanus cajan), and caballero (Caesalpinia pulcherrima) were screened for the presence of galactomannan. Only caballero tested positive for the presence of galactomannan. Isolation and purification of the galactomannan from caballero yielded an average of 5.59 % based on 10 grants of starting material. Analysis of the isolated polysaccharide with solutions of ninhydrin and iodine showed negative results indicating the absence of protein and starch. Water solution of the isolated polysaccharide also forms gel upon reaction with borax, which is known to form gels with galactomannan. Analysis of the viscosity of a 1.0 % solution of the isolate showed a kinematic viscosity of 24.25 centipoise and intrinsic viscosity of 1.063 dtJg, compared to standard guar gum which has 21.25 centipoise and 1.0 dUg, respectively. The isolated polysaccharide was hydrolyzed using GN hydrochloric acid for 15 hours at a temperature of 100°C. The neutralized hydrolyzate was subjected to thin layer chromatography on silica gel and yielded two spots with Rf values of 0.44 and 0.53, which correspond to the Rf values of standard D-galactose (0.45) and D-mannose (0.53).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2002 C4 O73

Document Type

Thesis

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