Genetic variability among rice (Oryza sativa L.) genotypes based on iron, zinc and phosphorous distribution in different plant parts and molecular markers

Date

4-2005

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Glenn B. Gregorio

Abstract

PALMA, MARINICA CIARA BONDAD, University of the Philippines Los Banos, Laguna, 2005 Genetic Variability Among Rice (Oryza Sativa L ) Genotypes Based on Iron Zinc and Phosphorous Distribution in Different Plant Parts and Molecular Markers. Advisers: Merlyn S. Mendioro and Glenn B. Gregorio

ABSTRACT

The genetic variability of iron, zinc and phosphorous distribution among eight genotypes of rice (Oryza sativa L.) namely Zuchein, Xua Bue Nuo, PSBRc28, 1R72, IR62266-42-6-2, 1R68144-213-2-2-3-1, 1868537-23-1-5-1 and 1871684-36-3-3-2 was determined. Three replicates for each variety were grown until maturity in culture solution under controlled environmental condition. The plant parts were then separated prior to micronutrient analysis. Results showed highly significant differences in the ability of the eight genotypes of rice to allocate Fe in the roots, rachis, stem, leaves, grain and hull. The same was true with Zn except for the roots. Fe was mostly distributed in the roots for all varieties, followed consecutively by the leaves, stem, rachis, hull and grain except for Xua Bue Nuo and IR68144-2B-2-2-3-1 in which there was higher Fe in the stem than in leaves. Strong linear relationships existed among these parts. Unlike in Fe distribution, the part of the plant with highest Zn concentration was dependent on the variety. P which is correlated with phytic acid is least in the hull and highest in stem. This could then be attributed to the presence of genes responsible for both absorption of individual micronutrients and for their transport within the plant. Also, strong linear relationships existed among these micronutrients. Genetic variability and phylogenetic relationship between lines were determined by employing 102 molecular markers (microsatellite), 58 of which had a polymorphism information content (PIC) value of more than 0.5. The 8 genotypes segregated into 2 major clusters, one of which constituted IR71684-36-3-3-2 and Zuchein. The highest coefficient of similarity generated was 0.5 which was composed of relatively two low-Fe varieties namely IR62266-42-6-2 and PSBRc28. Moreover, IR72 was similar to these varieties by 42%.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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