The effect of some antibiotics on the growth of a blue-green alga, Chroococcus turgidus Nageli

Date

3-1990

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Milagrosa, R. Martinez

Abstract

The growth response of Chroococcus turgidus to some antibiotics such as streptomycin sulfate. Penicillin-G and tetracycline was investigated using Tañada’s formulation as the basal medium.

The growth parameters used were dry weight, chlorophyll and protein analyses. The cultures were maintained in their axenic condition throughout the study.

The concentrations of the different antibiotics were the following control, 100 ug/ml, 750 ug/ml and 1000ug/ml. It was observed that the maximum concentration which allowed optimum growth of the organism was at 250 ug/ml for all the antibiotics. Analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) revealed that the effect of the three lower concentrations (0 ug/ml and 250 ug/ml) were not significantly different from one another. Similarly, the three higher concentrations (500 ug/ml, 750 ug/ml were not significantly different from each other.

Death of the organism was manifested for the concentrations higher than 250 ug/ml. Presence of empty cells was seen under the microscope. The liquid cultures were yellowing and there was no observed growth in agar slants.

In this study, it was proven that the cells of C. turgidus were sensitive to the antibiotics in the range of 250 ug/ml-500 ug/ml.

Person’s correlation coefficient showed that the growth parameters used (dry, chlorophyll and protein content) were significantly correlated to one another.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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