Isozyme polymorphism of two species of rice black bug, Scotinophara sorsogenensis (Barrion, et al.) sampled from Laguna and Scotinophara coarctata (Fabricius) from Palawan

Date

4-2008

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Isozyme analysis through starch gel electrophoresis was done on two rice black bug (RBB) (Scotinophara spp.) species — S. sorsogonensis from Aplaya, Calamba, and S. coarctata from two localities of Palawan (Magbabalot, Brookes Point and Tagbabalat, Espanola). Considering the three (3) enzyme systems, namely, acid phosphatase (ACP). alkaline phosphatase (ALP), and esterase (EST), nine presumptive isoloci were observed — ACP-I. ACP-2, ACP-3, ALP-I, ALP-2, ALP-3. EST-I, EST-2, and EST-3. Intraspecific and interspecific genetic variations in populations of Scotinophara species were revealed by the proportion of polymorphic loci (P), average number of allele per locus (A), average heterozygosity (II), genetic identity (1), genetic distance (D), and genotypic similarity (S). Computed values of P=55.6%, A=2, and H=20.8% for the Calamba RBH's are higher than those in two localities of Palawan; Magbabalot, Brookes Point with P=33.3%, A=1. and H=16.7%. and Tagbabalat, Espanola with P=0%, A=I. and H=0%. On the other hand, the computed genetic identity between the two localities of Palawan was higher (73.1%) than that between the Palawan and Calamba population (57.6%). These were consistent with the computed genetic distance (D), as the D between the two localities of Palawan was lower (31.3%) than that between Palawan and Calamba population (55.2%). Genotypic similarity also gave the same result as in genetic identity. These calculations imply that the samples from the two localities of Palawan are more related to each other and less related to samples from Aplaya. Calamba. NTSYS program was used to confirm these calculations and to show how the individual samples clustered. At —0.54 similarity index coefficient, the S coarctata samples were contained in one cluster, separated from the S. sorsogonensis samples.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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