Monitoring the sugar composition and biomass and biomass in sweet sorghum [Sorghum bicolor (L.) Moench] stalks at "Near maturity period"

Date

10-2008

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Veronica P. Migo

Abstract

ROMASANTA, RYAN R. College of Arts and Sciences, University of the Philippines Los Banos. October 2008. MONITORING THE SUGAR COMPOSITION AND BIOMASS IN SWEET SORGHUM STALKS AT "NEAR MATURITY PERIOD".

Adviser: Veronica P. Migo, Ph. D.

Bioethanol had been known to be a good substitute for the fast depleting fossil fuels. The Juice from sweet sorghum [Sorghum bicolor (L.) Moench] stalks contain high amount of sugars that can extracted and converted to bioethanol.

Brix degree was determined using a hand refractometer. Among the four varieties, SPV 422 showed the highest value of brix degree reaching up to 20° at Day 102 followed by 1CSR 93034, ICSV 93046 and ICSV 700. Translocation in plants plays an important part to understand all the trends and results produced from this study.

Total sugar content of sweet sorghum (Sorghum bicolor [L. Moench]) varieties was analysed using the Phenol-Sulphuric Acid Method and was measured using a UV-VIS spectrophotometer. SPV 422 has the highest amount of total sugar content produced having values ranging from 15.24 to 18.25 % follow.' by ICSR 93034, ICSV 700 and ICSV 93046. The values of the total sugar content are directly proportional with the brix degree values.

Dinitrosalicylic (DNS) Acid Method was used for the analysis of reducing sugars and was measured using a UV-VIS spectrophotometer. % TRS:TS was also calculated. ICSV 93046 showed the highest value for both parameters with values ranging from 1.56 to 4 .40% and 36 to 80% respectively, making it. This was followed by ICSV 700, ICSR 93034 and SPV 422. ICSV 93046 is the best variety of sweet sorghum for bioethanol production among the four in terms of total reducing sugar and % TRS/TS. Day 102 showed the best results among the best days of harvest. In regards with the results, the trends produced for both parameters shows high similarity with each other.

Correlation for the brix degree and total sugar content of sweet sorghum juice was conducted to verify if they had significant linear correlation. Other relationships were also studied and they are the following; total reducing sugar versus brix degree, total reducing sugar against total sugar content, and %IRS/TS versus brix degree. SPSS, a type of statistical software and Microsoft Excel was used for the analysis. Homogeneity of regression coefficients was done to determine if the data within a given relationship are valid to pool out.

For total sugar content (TS) versus brix degree, Day 102 gave the highest correlation coefficient giving a value of 86.35. For total reducing sugar (TRS) versus brix degree, Day 102 has the highest correlation coefficient of 62.74. For TRS versus TS, Day 195 showed the highest correlation coefficient of 70.99. For % TRS/TS versus brix degree, Day 95 showed the highest correlation with value of 73.09. All these correlations are valid only for varieties ICSV 93046 and ICSV 700. Brix degree showed positive correlation with total sugar content and total reducing sugar but inversely proportional with % TRS/ TS. Results and figures provided important information regarding the sends and values but the equations are not conclusive enough to prove that there is a strong correlation between the parameters.

Crude fiber content was analyzed using Fibertech 1020 Hot Extractor and 1021 Cold Extractor ICSR 93034 has the highest value of crude fiber content with 37.28% followed by ICSV 93046 (35.44 %). ICSV 700 (32.89%) and SPV 422 (31.41%).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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