Biology of Rice Striped Stemborer (RSS) Chilo suppressalis (Walker) and evaluation of the effects of Chymotrypsin inhibitor on the toxicity of Bacillus thuringiensis (B.t.) delta- endotoxins

Date

4-1993

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Cesar G. Demayo

Abstract

Insect colonies obtained from Ifugao and IRRI and their hybrids were mass reared under laboratory conditions. Developmental period, pupal weights, and sex ratio were monitored and observed to differ. Inheritance exhibited by the hybrids is maternal in origin and Chi-square test showed that saw ratio deviated from the hardy-Weinberg 1:1 sex ratio.

Behavioral responses of the larvas in the choice test showed the Chymotrypsin and Trypsin-Chymotrypsin inhibitors (Proteinase Inhibitors) inhibit first few days of feeding as opposed to B. t. where feeding is followed by sudden decrease, as B.t. plus proteinase is observed to inhibit even on the first day of exposure. Diet consumption, larval and pupal weights are least in the B.t. plus proteinase inhibitor treatments, likewise in the pupation rate. Mortality test increased death tolls in the pupation rate. Mortality test increased death tolls in the B.t. plus proteinase inhibitor. Results showed that toxicity of B.t. increase with the presence of proteinase inhibitor and as much as an effective alternative for insect control.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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