Further studies on the in vitro culturing of human red blood cells in coconut protein based media

Date

3-1980

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

In vitro culturing of human red blood cells in a coconut, protein base,: media within a 192-hour period showed significant viability by morphological and dye-exclusion, indices at the lower levels of CPI, specifically at and 2, CPI, respectively. The media prepared from the use of coconut water from nine-month old coconuts as solvent for the dissolution of CPI show indications to perform better than media prepared from HBSS. In fact, by statistical analysis, it was shown that HBSS decreases efficiency at the 168th and abruptly at the 192nd . whereas coconut water maintains its efficiency up to the 192nd hour. In the some study, prolonged survival of . blood cells was successfully undertaken to determine the efficiency of pure coconut water (0% CPI) as a culture media . It is concluded that pure coconut water could maintain red blood cells in vitro up to the 288th hour. Further studies are therefore suggested to focus on the use of the pure coconut water as a media for culturing red blood cells.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS