Morphological, anatomical and cytological characterization of friable and compact calli of carrot (Daucus carota L.)

Date

3-1991

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

To induce callus formation, root of carrot (Q. carotA) was cultured in Murashige and Skoog's medium (MS) with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and coconut water (CW). Callus of carrot was induced in all the treatments; MS, MS+0.1 ppm 2,4-D, MS+0.2 ppm 2,4-D, MS+ 10% CW, MS+20% CW, MS+10% CW+ 0.1 ppm 2,4-D, MS+ 20% CW + 0.1 ppm 2,4-D, MS+ 10% CW + 0.2 ppm 2,4-D, and MS+20% CW + 0.2 ppm 2,4-D. Two types of carrot calli, friable and compact, were observed after 3 months. Morphologically, the friable carrot callus is soft or loose while the compact carrot callus is nodular or tumorous. The color of either friable or compact carrot callus is light green, white or light orange. Histological sections of 3-month old friable and compact calli of carrot were obtained using plant microtechnique. The cells of friable carrot callus were larger than the cells of compact carrot callus. The cells from friable and compact carrot calli were obtained using cytological technique. The cells of friable and compact carrot calli have significant differences in their length but not in their width.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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