Comparative esterase banding patterns of three strains of Trichoderma harzanium Rifai (Hyphomycetidae : Moniliaceae)

Date

6-1994

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Presidena A. Baculod

Abstract

Three strains of Trichoderma harzanium, namely 36 and 72 for the parentals and a regenerant (REG) were examined for the enzyme esterase using starch gel electrophoresis. Several modifications were made in the trial run to intensify the presence of bands. These modifications are a) shaker (150 - 200 rpm) was used to increase the growth of L harzanium, h) the cultures were centrifuged for 20 minutes at 10,000 rpm and at 4°C to collect the mycelia, c) a glass homogenizer was used to boost the crushing of the mycelia, and d) the samples were freeze dried to get more concentrated extracts.

Electrophoretic pattern showed only one band for esterase. The parental strain 36 had an average Rf value of 0.462 while 72 had an average Rf value of 0.349 and the regenerant strain had an average Rf value of 0.484. All bands exhibited different intensities ranging from barely detectable to highly intense. Statistical analysis showed no significant differences between 36 and REG strains, but showed significant difference between 72 and REG strain.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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