"Amylase activity and electrophoretic variation of B- amylase isoenzyme in Saccharum officinarum (var. Caña azul), Saccharum spontaneum (Clone S.E.S. 84 A) and their hybrids"

Date

11-1984

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M. Engle

Abstract

An initial study confirmed the presence of active amylase in extracts from leaves of Cana Azul (Saccharum officinarum), SES 84A (Spontaneum) and their hybrids. Amylase specific activity showed highly significant variation between parents and hybrids, and among hybrids themselves. No apparent relationship between amylase specific activity, chromosome number and brix were found. /-amylase isoenzymes can be separated on starch containing gels (SCG) using polyacrylamide disc gel electrophoresis. Zymograms were ascertained for the parents and their hybrids. Cana Azul yielded 6 cathodic /-amylase bands while the remaining materials had 4 distinguishable cathodic /6-amylase bands each. Caña Azul was electrophoretically distinct from SES 84A by the presence of B2 and B3 zones in the former. Four common bands were shared by the parents signifying genetic homology and phylogenetic relationship between the cultivated species (Cana Azul) and the wild primitive relative (SES 84A). Some parental bands were absent in the hybrids and/or changed in staining intensities suggesting n- and n+ chromosome transmissions from the E. officinarum parent consistent with the aneuploid behavior of its genome. No obvious correlation between the electrophoretic band pattern and sucrose yielding capacity (brix) and chromosome number was found. The nature and behavior of transmission in each hybrid of two isoenzyme bands (81 and B4) were inferred; others cannot be ascertained. Possible causes of variability in amylase specific activity and electrophoretic profile were discussed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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