Isolation of the antimicrobial substances in rambutan (Nephelium lappaceum Linn.) leaves

Date

3-1984

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ann Maureen R. Rouhi

Abstract

Tan, Elizabeth T. College of Agriculture - College of Arts and Sciences, University of the Philippines at Los Banos, March, 1984. Isolation of the Antimicrobial Substances in Rambutan (Nephelium lappaceum Linn.) Leaves.

Advisers Dr. Ann Maureen R. Rouhi

The antimicrobial substances in the leaves of Nephelium lappaceum Linn., were investigated. Preliminary experiments were conducted to determine the optimum conditions for extracting the active substances. The method that was established involves extraction at room temperature of the sun-dried powdered leaves with methanol for 24 hours. The crude extract was fractionated by a rapid column chromatographic method by successive elution with solvents of increasing polarity. Eluates with inhibitory activity against the test organisms (Staphylococcus aureus, Escherichia coli and Saccharomyces cerevisiae) were further purified by semi-preparative thin layer chromatography. The inhibitory components in the chromatograms were determined by testing the eluted bands on the test organisms.

Nine antimicrobial substances were isolated from the 11 column eluates and found to be active at a concentration of 0.68 gm/ml.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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