Characterization of the National Institute of Molecular Biology and Biotechnology (BIOTECH) Saccharomyces cerevisiae isolates with potential industrial applications.

Date

4-2012

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Co-adviser

Jessica F. Simbahan

Abstract

A total of three Saccharomyces cerevisiae isolates (2008, 2013 and TB3). their 26 mutants and fusants generated by gnome shuffling, together with the industrial strain 2030, from the National Institute of Molecular Biology and Biotechnology (BIOTECH) were characterized based on cell volume, ethanol tolerance, therniotolerance, trehalose content and total protein handing pattern. Two other methods namely, ploidy level determination and DNA microsatellite fingerprinting were also attempted. Results showed that there was no significant trend in cell volume of the different strains considered. Higher cell volume was expected after every round of protoplast fusions however, this was only true for the F1 of 2008. Based on ethanol tolerance, second generation fusants of 2008 were better than the parents and F1 while there was loss in ethanol tolerance of the TB3 F2s and 2013 mutants. There was a decrease in growth of strains in increasing temperature. The parent strains, TB3 and 2013, were more thermotolerant compared to their fusants mutants. On the other hand, some 2008 fusants were more thermotolerant than 2008. F1M52 was remarkably thermotolerant at 40°C. Trehalose content was measured. Duncan Multiple Range Test result showed that there was no correlation observed between trehalose content and ethanol tolerance or thermotolerance. There were also no detectable differences among protein banding patterns of the different strains. In general, almost all strains were observed to be highly ethanol tolerant (20% v/v).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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