Variation and inheritance of seed proteins in mungbean, Vigna radiata L. Wilczek x Vigna radiata subspecies sublobata.

Date

3-1992

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Desiree M. Hautea

Abstract

Sodium polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the degree of seed protein polymorphism of Vigna radiata c.v. VC3890 and Vigna radiata ssp. sublobata Acc. TC1966 and the mode of inheritance of polymorphic seed polypeptides on the F2 progeny of the cross between the two mungbean lines. Four polymorphic loci were studied and their patterns of segregation were established. Locus 1, which is composed of a 63 kd polypeptide type found in VC3890 exhibited a null phenotype in TC1966. Segregation analysis of F2 progeny revealed a significant deviation ratio from a 3:1 ratio expected of dominant gene action. Locus consisted of a 59.6 kd type in VC3890 and a 61.4 kd type in TC1966 showed inheritance patterns of 1:2:1 typical of codominant alleles. Loci 3 and 4 consisted of both null forms for VC3890 and a 40.6 and 28.2 kd types respectively in TC1966 which segregated in 3:1 ratios typical of single dominant alleles for both of the null form. The identities of these polymorphic polypeptides were also proposed.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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