Genetic variability of eight rice (Oryza sativa L.) genotypes based on grain iron content, physical parameters and microsatellite markers

Date

4-2005

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Glenn B. Gregorio

Abstract

Eight rice (Oryza Sativa L.) genotypes were grown and evaluated for genetic variability in iron content in the grain Results showed that as milling time increased, iron content in grains decreased along with total milled rice and head rice. Grain whiteness, transparency, and milling degree increased with milling time. Statistical analysis using two- factorial strip plot design showed that the effects of variety, milling time, and interactions of both were highly significant. IR71684-36-3-3-2, IR68144-2B-2-2-3, and IR76528-18-2-2-2-2 had the highest iron content for all milling times. Genetic variability observed in the genotypes suggest that combining the desirable grain characters of improved varieties like IR64 with high iron content in grains is attainable. Histochemical localization revealed the higher concentration of iron in the embryo, especially in the epithelium- scutellum and aleurone layers of all rice genotypes tested, relative to iron in the endosperm The intensity of the blue color reaction in the embryo of different rice genotypes was indicative of their grain iron content for both brown and milled rice Those with high grain iron like IR68144-20-2-2-3, 1876528-18-2-2-2-2, and 1R71684-36-3-3-2 were distinguishable from those with low iron genotypes like Azucena and IR74796-3B-34-1-1 DNA fingerprinting was done to evaluate the degree of polymorphism and genetic variability that exist among the eight genotypes using simple sequence repeats (SSR) or microsatellites. Polymorphism Information Content (PIC) values ranged from 0.38 to 0.84 with a mean of 0.60. Cluster analysis showed a 17% overall similarity of the 8 genotypes with two main clusters due to common parentage.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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