Voltametric determination of lead (II) using a carbon paste electrode modified with egg albumin

Date

10-2005

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Elmer Rico E. Mojica

Abstract

SIA, CHRISTINE JOY V, College of Arts and Sciences, University of the Philippines Los Banos, April 2005. Voltammetric Determination of Lead (H) Using a Carbon Paste Electrode Modified with Egg Albumin.

Adviser: Elmer Rico E. Mojica

A novel carbon paste electrode (CPE) modified with egg albumin was investigated for voltammetric behavior prior to and after lead accumulation. The functional groups of the side chains of the amino acids present in egg albumin such as histidine, aspartic acid and glutamic acid, cysteine, methionine, swine, threonine, tyrosine, tryptophan, arginine, asparagine, and glutamine provided the binding sites for metal adsorption. In effect, egg albumin has the ability to form complexes with the metal ions. Preliminary investigation demonstrated the ability of the egg albumin modified CPE to accumulate lead (II) ions on its surface and generate peaks within -400 to -600 mV range. Optimization of the experimental parameters for the analysis was conducted. The optimum parameters were 15% modifier, 0.1 M HCI as supporting electrolyte, 3 minutes accumulation, pH 7 of the accumulating solution, 15 seconds deposition time, and -1000 mV deposition potential. The selectivity of the egg albumin modified CPE was investigated by studying the interferences from other metals. Several metals were tested as analyte such as Cd II), Cu (II), Co (11), and Hg (II), which showed peaks at their respective potential regions. Regeneration of the electrode surface was performed using multiple stripping and dipping in EDTA. The egg albumin modified CPE gave a sensitivity of 0.05 pA/ppm, limit of detection (LOD) of 0.52 ppm, and limit of quantification (LOQ) of 1.75 ppm. For the practical application, analysis of the laboratory waste sample was done using the optimized conditions. The lead concentration of the laboratory waste sample using DPASV is 2.26 ppm, while the flame AAS gave a 5.20 ppm concentration.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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