Comparative analysis of glucose-6-phosphate dehydrogenase isozymes among malaria infected and non-infected residents of Nagcarlan, Pakil, Pangil, and Los Baños, Laguna

Date

11-1994

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

Starch gel electrophoresis for glucose-6-phosphate dehydrogenase (G6PD) showed polymorphism among 21 malaria infected residents and 98 non-infected residents from Nagcarlan Pakil, Pangil, and Los Banos, Laguna. For the malaria infected residents. 4 G6pd isozymes were observed namely, G6pd-la, G6pd-lb, G6pd-2a and G6pd-2b with Rf means of 0.07, 0.16, 0.43 and 0.50, respectively. One malaria infected resident did not show any visible band. Among malaria infected residents, a total of five genotypes were observed, namely G6pd-laa, G6pd-lbb, G6pd-lab, G6pd-2aa and G6pd-2bb with genotype frequencies of 0.10, 0.70, 0.20, 0.60 and 0.40, respectively. For the non-infected population, 5 G6pd isozymes were observed namely, G6pd-la, G6pd-lb,G6pd-lc, G6pd-2a and G6pd-2b with mean Rf values of 0.08, 0.16, 0.24 0.43 and 0.50, respectively. Of the 98 non-infected residents, 14 did not show any visible bands. Among non-infected residents a total of seven genotypes were observed namely, G6pd-laa, G6pd-lbb, G6pd-lcc, G6pd-lab, G6pd-lac, G6pd-2aa and G6pd-2bb with genotype frequencies of 0.07, 0.54, 0.15, 0.14, 0.10, 0.74 and 0.26, respectively. The total population was in disequilibrium for both loci G6pd-I and G6pd-2 but the set of samples taken from the four areas of Laguna were in equilibrium when tested individually, except for the set of samples from Nagcarlan relative to G6pd-1 and the set of samples from Pangil relative to G6pd-2. Between the populations from the four areas of Laguna, genetic identity values were high at I = 0.5556 to 0.9478 and consequently genetic distance values were small at D = 0.0536 to 0.2656 except for that between Pangil and Nagcarlan which had relatively high genetic distance as compared to the other areas.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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