Biosorption of CU2+ using immobilized Rhizobium cells (BLI 80)

Date

4-1994

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Juanita C. Mamaril

Abstract

Rhizobium loti strain BL1 B0 was used to reduce the Cu (II) concentration of a laboratory prepared 15 ppm Cu (II) solution. The strain was grown in yeast extract mannitol agar (YEMA) and harvested after 4-5 days of incubation. An average of 9.0 ppm Cu (II) was adsorbed by 0.5 g (wet weight) of the cells.

A biotrap consisting of immobilized cells was developed and was able to reduce Cu (II) concentration effectively. The cells grown in yeast extract mannitol broth (YEMB) were imbibed into the pores of ceramic beads. Optimum flowrate of the biotrap was determined to be 0.3 ml/minute with an average of 6.5 ± 0.50 ppm adsorbed.

The pH of the influent used to desorb the trapped Cu (II) ions affected the efficiency of desorption. HC1 solutions of pH 2.92 and 4.0, and deionized water (pH55-7) were passed through the biotrap. Higher efficiency was obtained at lower pH of HC1 solutions.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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