Genetic differentiation of four garlic clones, Allium sativum L., based on Amplified Fragment Length Polymorphism (AFLP)

Date

4-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Four garlic, Allium sativum L., clones (G143 from Brazil; TA459 from Japan; G91 from India and; G76 from Dominican Republic) previously classified belonging to the four RAPD groups (I-IV) were subjected to Amplified Fragment Length Polymorphism (AFLP). Twenty-seven selective amplification primers were subjected to different pre-amplification primers, EcoRI-A/Msel-C and EcoRI-AC and Msel-CC. Four EcoRI primers, E3 (+ACA), E4 (+ACT), E5 (+ACC) and E6 (+ACG) were used together with seven Alm' primers. Polymorphisms range from 22% - 95% in A/C, and 6%-94% in AC/CC. Twenty five primer combinations are shown useful in detecting ample amount of polymorphisms, EcoRI-ACG/Msel-CCG showed the highest (94.55%). AFLP reveals possible genetic markers for each clone. EcoRI-ACT/MseI-CCGC (E4/M6) for Clone I with three fragments (470, 325 and 190 bp), EcoRI-ACA/Msel-CCGC (E3/M6) for Clone II with two fragments (345 and 235 bp), EcoRI-ACT/Msel-CCG (E4/M4) for Clone III with six fragments (475, 480, 425, 380, 310 and 260 bp), and EcoRI-ACTIMsel-CCC (E4/M1) for Clone IV with three fragments (400, 310 and 250 bp). Jaccard Similarity Index was used to differentiate the four clones. Similarity coefficient ranges from 74.1%-77.4%. The high value of similarity coefficient indicates that the four clones are highly similar to each other.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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