Genetic characterization of pili (Canarium ovatum Engl.) (Burseraceae) isozyme analysis.
Date
4-2008
Degree
Bachelor of Science in Biology
Major Course
Major in Cell Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Merlyn S. Mendioro
Abstract
To genetically characterize the 30 accessions of pill, Canarium ovatum Engl. (11 unknown origin. 8 from Albay, 10 from Camarines Sur and 1 from Camarines Norte) starch gel electrophoresis was conducted. The four isozymes studied were mutate dehydrogcnase (MDH), esterase (EST), acid phosphatase (ACPH) and alkaline phosphatase (ALPH) from young actively growing leaves. No MDH enzyme activity was detected in any of the 30 accessions. Esterase has three zones of activity (EST-1, EST-2 and EST-3) with a total of 19 banding patterns. Four loci (ACPH-1, ACPH-2, ACPH-3 and ACPH-4) and 22 banding patterns were observed in ACPH. Twenty-four handing patterns and four loci (ALPH-1, ALPH-2, ALPH-3 and ALPH-4) were observed for ALPH. Considering all the 11 loci. all the accessions studied were different from each other. Only one unknown was identified to have similar banding pattern for EST, ALPH and ACPH with one accession collected from Albay. Other accessions of unknown origin need to be studied using other enzyme systems to establish their identity. Numerical Taxonomy and Multivariate Analysis System (NTSYS) failed to provide a definite cluster for all 30 accessions. Individuals of similar origin were grouped and subjected to POPGENE 32 analysis. Dendrogram showed that Camarines Sur and Albay populations belong to the same cluster while the sample from Camarines Norte is genetically distant from the two populations.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Velasco, Vera Marjorie E., "Genetic characterization of pili (Canarium ovatum Engl.) (Burseraceae) isozyme analysis." (2008). Undergraduate Theses. 12147.
https://www.ukdr.uplb.edu.ph/etd-undergrad/12147
Document Type
Thesis
