Morphological and isozyme analyses of natural populations of Bactrocera spp. (Diptera: tephritidae) from Guimaras Island and Laguna

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Ma. Genaleen Q. Diaz

Abstract

Morphological and isozyme analyses were used to determine if there were differences among the groups of Bactrocera spp collected from Guimaras Island and Laguna. Each population was divided into seven groups using six morphological criteria. Morphological analysis of aedeagustaculcus length and wing cell diameter showed that these did not differ considerably among the groups. Isozyme analysis revealed eight presumptive loci coding for three enzymes. Acid phosphatase and esterase had three loci each, while alkaline phosphatase had two. Two alleles. S and F, were found for ACP-1. ACP-3. EST-1, ALP-1, and ALP-2. ACP-2 and EST-3 showed one allele, S, while EST-2 showed three, S, M, and F. Genetic variation was high in the groups presumed to be B. occipitalis and low in the groups considered as hybrids. Genetic diversity was higher in groups from Guimaras Island. Interpopulation estimates of variation showed the presence of considerable genetic variation among the groups. Some of the groups can be categorized together and these categories were supported by the presence of unique alleles and by clusters obtained from a dendrogram. However, the patterns of groupings were not consistent between the two locations. It was found that the morphological data did not reflect the isozyme variation.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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