Isozyme analysis of selected species belonging to family Sapotaceae

Date

4-2007

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Co-adviser

Felipe S. Dela Cruz Jr.

Abstract

To assess genetic variation within and among four genera belonging to Family Sapotaceae, a total of thirty samples of chico (Manilkara zapota (I..) P. van Royen), caimito (Chrysophyllum cainilo L.), caimitillo (C. ohvifirme L.), sawo kecik (M. kauki (L.) Dubard), abiu (Pouteria caimito Radlk.), tiesa (P. campechiana (Kunth) Baehni), and miracle fruit (Synsepahtm dulcificum L.) were characterized using enzyme systems of acid phosphatase (ACPH), alkaline phosphatase (ALPH), esterase (EST), glucose 6-phosphate dehydrogenase (G6PD), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), and shikimatc dehydrogenase (SDH).The isozyme banding patterns showed that ACPH, ALPH, MDH, PGI, PGM, and SDII were all monomorphic in contrast to the polymorphic EST and G6PD. Seventeen presumptive loci were observed for the eight enzyme systems studied Fourteen loci were observed in both caimitillo and sawo kecik, 13 in chico, caimito and tiesa, 12 in abiu, and 11 in miracle fruit All of the bands were designated as SS, except for EST./ in all samples and G6PD-I in caimitillo and sawo kecik which were heterozygous for the fast and slow migrating band, FS. Clustering revealed the genetic closeness of the samples such that all belong to a single group at 0.66 similarity coefficient The samples studied had a narrow base of genetic variability among themselves

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS