Isozyme analysis of selected species belonging to family Sapotaceae

Date

4-2007

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Co-adviser

Felipe S Dela Cruz Jr

Abstract

To assess genetic variation within and among four genera belonging to Family Sapotaceae, a total of thirty samples of chico (Mani/kara :allow (L.) P. van Royer* caimito (Cluysopk•llum cainito L.), caimitillo (C. oliviforme L.), sawo kecik kauki (L.) Dubard), abiu (Pouteria caimito Radlk.), tiesa (P. campechiana (Kunth) Baehni), and miracle fruit (Symepalum dulcificum L.) were characterized using enzyme systems of acid phosphatase (ACPH), alkaline phosphalase (ALPH), esterase (EST), glucose 6-phosphate dehydrogenase (G6PD), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), and shikimatc dehydrogenase (SDH).The isozyme banding patterns showed that ACPH, ALPH, MDH, PGI, PGM, and SDFI were all monomorphic in contrast to the polymorphic EST and G6PD. Seventeen presumptive loci were observed for the eight enzyme systems studied Fourteen loci were observed in both caimitillo and sawo kecik, 13 in chico, caimito and tiesa, 12 in abiu, and 11 in miracle fruit. All of the bands were designated as SS, except for 1ST-I in all samples and G6PD-/ in caimitillo and sawo kecik which were heterozygous for the fast and slow migrating band, FS. Clustering revealed the genetic closeness of the samples such that all belong to a single group at 0 66 similarity coefficient. The samples studied had a narrow base of genetic variability among themselves.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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