Isolation, purification and partial characterization of a limonoid from calamansi (Citrus madurensis Lour.) peels and evaluation of its larvicidal activity against Aedes aegypti L. (Diptera : Culicidae) / Judy Anne Dimaculangan Alo ; Annabelle T. Abrera, adviser.

Date

12-2014

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Annabelle T. Abrera

Co-adviser

Kevin C. Salamanez

Committee Member

Marlon N. Manalo

Abstract

Limonoids were extracted from calamansi (Citrus madurensis Lour.) peels and from dalanghita (Citrus reticulata Blanco) seeds using methanol. The crude extracts were partitioned with 1:1 dichloromethane: water and were added with 2-propanol to crystallize the limonoids. The crystals were purified by dissolving it in dichloromethane and crystallizing it with 2-propanol. Thin layer chromatography suggested that the isolates were pure and could be similar, having similar retention factors. The isolates were also confirm.] to belong to limonoid group showing positive result of orange solution in Ehrlich's test. UV-Vis analyses of the two isolate., suggested the presence of furan and lactone. though in the isolate from calamansi peels, the peak is not defined but the highest absorbance was present at the expected wavelength. Comparison of melting point of the isolate from dalanghita seeds (280- 286") as well as from calamansi peels (278- 284°C) suggested that it could be limonin. FT-IR analysis of isolate from dalanghita seeds further confirmed that it is a limonoid, and possibly limonin. FT-IR analysis of isolate from calamansi peels suggested that it is possibly a limonoid containing 011 group. '11-NMR analysis further confirmed the presence of 011 group, thus, it was concluded that the isolate from the calamansi peels can possibly be limonol or ichangin. After the performed analyses. the isolate from the calamansi peels was used for a larvicidal toxicity assay against .A. aegipti L. It was estimated that the LC59 at 48 hours after treatment was 160.81 ppm and the LC,0 72 hours after treatment was 22.37 ppm. Through this bioassay, it was noticed that the isolate also affects the growth and development of A. aegypti., aside from its larvicidal activity. The wastes produced during the conduct of the study were kept in properly labelled waste bottles.

Language

Filipino

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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