Isolation and partial characterization of a lectin from the seeds of calamansi (xCitrofortunella microcarpa (Bunge) Wijnands)

Date

6-2000

Degree

Bachelor of Science in Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Milagros M. Peralta

Abstract

Lectin was extracted from Calamansi seeds using 0.02 M phosphate buffered saline solution at pH 7.4. Further purification was achieved by ammonium sulfate fractionation at 0-30% saturation and gel filtration chromatography using Sephadex G-200. Hemagglutination assay using human blood types was used to detect the presence of the lectin. The purified lectin is non-blood type specific because it can agglutinate all human blood types. However, it is animal blood group specific since it preferentially agglutinates carabao blood but not calf and goat erythrocytes. It is also classified as a complete lectin since it can agglutinate erythrocytes at physiological saline solution without trypsination. Hapten inhibition studies did not show any specificity towards the ten sugar standards tested. The lectin is a glycoprotein containing 0.0568% total sugars as determined by the phenol-sulfuric acid method. Polyacrylamide gel electrophoresis under non-denaturing conditions (native PAGE) yielded a homogeneous band indicating the possible purity of the lectin. SDS-PAGE revealed four sub-units with estimated molecular weights of 50.8 KD. 66.2 KD, 105 KD and 137 KD.

Language

Filipino

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2000 A13 A54

Document Type

Thesis

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