Wall-bound phenolics in the resistance of tomato (Lycopersicum esculentum Mill.) against the bacterial wilt pathogen (Ralstonia solanacearum)

Date

4-1998

Degree

Bachelor of Science in Agricultural Chemistry

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae T. Mendoza

Co-adviser

Nieva T Librojo-Basilio

Committee Member

Milagros M. Peralta

Abstract

The profile of wall-bound phenolics produced by susceptible and resistant tomato plant in response to Ralstonia solanasearum infection was determined. The wall-bound phenolic uniquely produced by the resistant tomato plant was also identified, isolated, and tested for its effect on the growth of Ralstonia solanacearum. Wall-bound phenolics were extracted from prepared cell wall materials by alkaline hydrolysis carried out at room temperature for 20 hours. The hydrolysate was extracted with ethyl acetate. evaporated, arid dissolved in methanol Extracted wall-bound phenolics from resistant and susceptible, control and infected, root, stem and leaf samples were run on thin layer chromatography using 97 chloroform. acetic acid solvent system Six unique bands were formed by the infected resistant tomato root and four of these bands were further separated when another run in thin layer chromatography was made using 3 I ethyl acetate. petroleum ether solvent system Bands nos 5, 6, 9, 10, 12, and 14, dissolved in DMSO, were tested for its antibiocidal activity. The average CFU/mL was significantly reduced by 2.8 to 66 7x upon treatment with the wall-bound phenolics. A reduction in colony size and a 24 hour-delay in bacterial growth were also observed

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1998 A13 G66

Document Type

Thesis

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